Antigen ELISAs for Trypanosomes

Evaluation of the Performance


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Table of Content


Antigen ELISAs for Trypanosomes
Evaluation of the Performance

Proceedings of a Workshop held at ILRI Nairobi, Kenya
9–11 December 1996

Edited by

Subhash Morzaria
Rachel Masake
John Rowlands
Tony Musoke

International Livestock Research Institute, P.O. Box 30709, Nairobi, Kenya

The International Livestock Research Institute (ILRI) began operations on 1 January 1995. ILRI is one of 16 centres in a worldwide agricultural research network sponsored by the Consultative Group on International Agricultural Research (CGIAR). ILRI conducts strategic research in the biological, animal and social sciences to improve livestock productivity in sustainable agricultural systems throughout the developing world.

The objectives of the institute’s research programmes are to improve animal health, nutrition and productivity (milk, meat, traction) in ways that are sustainable over the long term, to characterise and conserve the genetic diversity of indigenous tropical forage species and livestock breeds, to promote sound and equitable national policies for animal agriculture and natural resource management, and to strengthen the animal husbandry research programmes of developing countries.

This publication was typeset on a microcomputer and the final pages produced on a laser printer at ILRI, P.O. Box 30709, Nairobi, Kenya, telephone 254 (2) 630743, fax 254 (2) 631499, e-mail ILRI-Kenya@cgnet.com. Printed by ILRI, P.O. Box 5689, Addis Ababa, Ethiopia, telephone 251 (1) 613215, fax 251 (1) 611892, e-mail ILRI-Ethiopia@cgnet.com.

ISBN 92–9146–042–7

Correct citation: Morzaria S., Masake R., Rowlands J. and Musoke T. (eds). 1998. Antigen ELISAs for Trypanosomes. Evaluation of the Performance. Proceedings of a Workshop held at ILRI, Nairobi, Kenya, 9–11 December 1996.ILRI (International Livestock Research Institute), Nairobi, Kenya. 135 pp.


Table of Contents


Foreword

Diagnostic tests requirements, development, standardisation and validation

Requirements for diagnostic tests to improve field investigations into the epidemiology and control of trypanosomosis

Development, standardisation and validation of enzyme immunoassays: an integrated process

Ag-ELISAs for trypanosomes

Antigen-ELISA for detection of trypanosomes

Performance of a direct sandwich enzyme-linked immunosorbent assay for detection of trypanosomal antigens

Evaluation of Ag-ELISA in the field

Sensitivity and specificity of antigen-ELISAs for diagnosis of Trypanosoma congolense and Trypanosoma vivax infections in cattle

Evaluation of antigen-detection ELISAs in Mali

Importance of antigen-detection ELISA in monitoring and implementing control strategies for trypanosome infection in KwaZulu-Natal

Use of antigen-ELISA in monitoring and implementing trypanosomosis control strategies in Kenya and Uganda

Application of the antigen-detection ELISA test in the diagnosis of trypanosomal infections in ruminants in Burkina Faso

The use of enzyme-linked immunosorbent assays (ELISAs) for trypanosomosis surveys and surveillance

Validation and use of Ag-ELISAs in the diagnosis of Trypanosoma evansi: infections in buffalo in Indonesia

Other diagnostic assays Ab-ELISA and DNA-based test

Antibody detection tests for diagnosis of African trypanosomosis

Nucleic acid-based systems for detection of trypanosomes

Summary of discussion groups

Recommendations

List of participants