African trypanosomes. Cultivation of animal infective Trypanosoma brucei in vitro

Journal Article
Published on
byAmerican Association for the Advancement of Science

Abstract

Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37°C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces.

Citation

Science;196: 992-994

Authors

  • Hirumi, H.
  • Doyle, J.J.
  • Hirumi, K.

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