A highly sensitive, non-radioactive assay for T cell activation in cattle: Applications in screening for antigens recognised by CD4+ and CD8+ T cells

Journal Article
Published on
byElsevier

Abstract

We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFN y and activation of immune CD4 - and CD8 - T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4-T cell clones.

Citation

Journal of Immunological Methods;239: 85-93

Authors

  • Ballingall, Keith T.
  • Mwangi, David Miano
  • MacHugh, Niall D.
  • Taracha, E.L.N.
  • Totte, P.
  • McKeever, Declan J.

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