ILRI Forage Genebank

ILRI Forage Genebank

The International Livestock Research Institute (ILRI) Forage Genebank operates within the Livestock Genetics, Nutrition and Feed Resources program, with the core mandate to conserve, distribute and promote the use of diverse forage genetic resources. It holds an extensive and diverse collection of forage accessions and related information. By safeguarding these resources, the genebank contributes to sustainable development goals on biodiversity conservation, food and nutrition security, and climate adaptation. It also contributes to CGIAR programs such as Sustainable Animal and Aquatic Foods, Breeding for Tomorrow, Sustainable Farming, Multifunctional Landscapes, and Better Diets and Nutrition, where genetic resources play an essential role.

Forage collection

Openly available as part of a global system of genetic resources conservation and sustainable use, the ILRI genebank in Addis Ababa, Ethiopia, conserves approximately 19,000 accessions of over 1,500 forage species. This represents one of the most diverse collections of forage grasses, legumes, browses and fodder tree species held in any genebank globally and includes the world’s major collection of African grasses and tropical highland forages.

Since 1994, ILRI’s germplasm collection has been held in trust under the auspices of the Food and Agriculture Organization of the United Nations (FAO) as part of an international network of ex situ collections. In October 2006, the institute signed an agreement to include this genetic material under the International Treaty on Plant Genetic Resources for Food and Agriculture (ITPGRFA). ILRI claims no ownership nor seeks any intellectual property rights over the germplasm and related information.

Conservation methods

In the active and base genebank collections, seeds are stored in laminated aluminium foil packets at 5 °C for medium-term needs (such as research and distribution) and at –20 °C for long-term preservation. Seed quality is ensured through initial viability and seed health testings as well as through continuous viability monitoring while in storage.

ILRI also manages field genebanks across Sodo, Zwai, Bishoftu and Addis Ababa in Ethiopia for forage species that rarely produce seeds or whose seeds are short-lived. Seed safety duplicates are maintained at the Alliance of Bioversity International and CIAT’s Future Seeds in Colombia and the Svalbard Global Seed Vault in Norway.

Quality management system

The genebank has established and implemented a quality management system to ensure compliance with FAO standards and other international guidelines for conservation of genetic resources. Standard operating procedures are used for the regeneration, conservation, and viability testing and monitoring of seeds.

To ensure the production of clean and healthy seeds, a dedicated germplasm health unit oversees the testing of samples and supports monitoring and sanitation management of plants under greenhouse and field conditions.

Database management system

A comprehensive data management and information system supports effective management of the germplasm collection. The data maintained include passport data, germplasm inventory, viability, health status, and characterization datasets. These records are systematically curated, updated, and linked to global platforms such as Genesys PGR, the FAO Global Information System, and the Multilateral System of Access and Benefit-sharing of the International Treaty on Plant Genetic Resources for Food and Agriculture. To enhance accuracy, tracking, and long-term traceability, each accession is assigned a digital object identifier (DOI).

The system is further enhanced by integration of barcoding in the laboratory and field, cloud-based mobile data collection via Kobo-Collect, and extensive imaging resources mapped to Genesys.

Information management standard operating procedures provide a clear framework that ensures consistency, accuracy, and efficiency in data management.

 

 

Research activities

Poor-quality feed and fluctuating supply, mainly associated with seasonal feed shortages, hamper livestock productivity in many tropical countries. Understanding and managing forage diversity is essential for the development of new forage genetic resources to alleviate these constraints and to maintain diversity in forage/pasture ecosystems.

At the genebank, research focuses on the agronomic performance of forage resources and their potential use as livestock feed. This involves assessing variation in phenotypic and nutritional traits, climate adaption and resistance to pests and diseases. Information generated from this research is used to identify superior accessions or “best bets” for further agronomic evaluation and utilization as part of sustainable farming systems.

Beyond their uses as feeds for livestock, forages play a key role in nature. They have been shown to improve soil fertility through nitrogen fixation/leaf drop; reduce erosion through increased ground cover, especially on slopes; help control insect pests; and provide environmental services (such as enhanced biodiversity, carbon sequestration and water productivity) leading to improved system resilience. They are also an important land use strategy for marginal lands and steep slopes that are not suitable for crop production.

 

 

Subset and core collection development

In collaboration with other projects, the genebank has characterized and evaluated a number of forage species. The generated data and information were used to develop subsets and best-bets of tropical and sub-tropical forage species, including accessions for drylands; accessions for highlands; a lablab core collection; promising disease resistant/tolerant Napier grass accessions ; and promising drought-resistant Rhodes grass accessions. These collections provide a valuable resource for further research, breeding programs, and the development of resilient forage production systems tailored to specific environmental conditions.

Distribution

Each year, the ILRI genebank distributes seeds, cuttings and root splits to requestors who agree to the terms of the ITPGRFA standard material transfer agreement for evaluation and further development and use by smallholder farmers. So far, hundreds of thousands of samples have reached users in more than 115 countries.

Researchers, farmers, research institutions, universities, and other interested parties can access germplasm information and request seeds online through the genebank’s website, Genesys and/or GRIN-GLOBAL .

Research Facilities

The genebank has advanced research facilities and laboratories to support forage research on seed processing, germination, taxonomy, disease diagnostics, molecular characterisation and evaluatio:

  • Field sites in Shola, Bishoftu, Zwai and Soddo are used not only for regeneration of seeds, but also for the conservation of live plants, especially species that do not produce seeds or produce short-lived seeds. The sites are used to screen collections to identify promising lines for further evaluation.
  • Seed processing and conservation facilities ensure that samples are prepared and stored under optimal conditions.
  • A dedicated germination laboratory, equipped with various incubators and facilities for seed pretreatment, conducts germination tests to assess the viability and longevity of seeds.
  • Herbarium and taxonomic resources are used to study and classify plant species, contributing to the accurate identification and documentation of genetic resources.
  • Laboratories and greenhouses in the germplasm health unit support the diagnosis and identification of plant diseases and seedborne pathogens. This ensures that the genebank produces, conserves and distributes pathogen-free seeds.
  • A molecular laboratory for the genetic characterization and evaluation of plant materials supports advanced research in plant breeding and conservation.
  • The genebank also collaborates with the ILRI nutrition lab to generate and understand the feed quality of the forage species, aiding in the selection and improvement of forage varieties with better feed quality.

These facilities are also accessible to other projects and partners on a cost recovery basis, ensuring that a wider scientific community can benefit from the genebank’s resources while maintaining operational sustainability.

Capacity building

The genebank is committed to strengthening the capacity of national partners, visiting scientists, and students through comprehensive training programs and development of traning manuals. These programs cover key areas such as genebank and germplasm management, seed production, seed quality testing, germination, seed health testing, molecular techniques, data management and analysis.

Partners

Ethiopian Biodiversity Institute

Ethiopian Biodiversity Institute

Ethiopian Agricultural Authority

Ethiopian Agricultural Authority

Ethiopian Institute of Agricultural Research

Ethiopian Institute of Agricultural Research

Ministry of Agriculture

Ministry of Agriculture, Ethiopia

Funders

Federal Ministry for Economic Cooperation and Development

Global Crop Diversity Trust

CGIAR Trust Fund

CGIAR Trust Fund