
Genomics platform
Services
Molecular biology
Molecular Biology Techniques provide opportunity to study the composition, structure and interactions of cellular molecules. These techniques include DNA cloning, bacterial transformation, transfection, chromosome integration, cellular screening, Nucleic acid extraction, Nucleic acid amplifications and synthesis (PCR and cDNA synthesis) DNA sequencing, molecular hybridisation, random mutagenesis, among others. We offer a portfolio of the most essential techniques as well as many custom molecular engineering technologies.
Library preparations
Library preparation forms the basic initial stage of NGS. Prior to sequencing nucleic acid from samples, DNA or RNA must be extracted, fragmented, end-repaired, and covalently attached to adapters using ligation or tagmentation processes. The standard and quality of NGS library preparation determines the accuracy of NGS data.
RT-qPCR analysis
qPCR and RT-qPCR are now widely used in a range of applications such as gene expression analysis, SNP identification, genotyping, contamination screening, and molecular diagnostics for infectious diseases. An optimised protocol with standardised step by step
Nucleic acid extractions
Nucleic acid extraction processes include isolation, purification, concentration and quantification. Within our platform we have developed protocols for RNA and DNA extraction for a wide range of nucleic acid extraction kits. We perform extraction using high throughput automatic extraction machines such as Tanbead (Fig. 9), semi-automatic processes with column-based technologies and also manual (gold standard) lysis and precipitation techniques described in Maniatis laboratory manuals.
SEQUENCING PLATFORMS

High throughput sequencingtechnologies (HTST) have been build using a combination of expertise from different fields of science including chemistry, molecular biology, software, and engineering. Most of the current HTST (except for the Oxford Nanopore technology) have been built upon the principle of the Sanger’s Nobel prize winning discovery of dideoxynucleotide chain termination reaction.
Genomics Team
