Novel baculovirus-derived p67 subunit vaccines efficacious against East Coast fever in cattle

Journal Article
Published on
byElsevier

Abstract

Two novel baculovirus-derived recombinant Theileria parva p67 constructs were tested for their vaccine potential against East Coast fever. Boran calves were immunized with a his-GFP-p67 fusion protein (GFP:p67DeltaSS) or with GP64:p67C, a protein fusion between a C-terminal domain of p67 and the baculovirus envelope protein GP64. Both GFP:p67DeltaSS and GP64:p67C induced antibodies with high ELISA titers that neutralized T. parva sporozoites with high efficiency. Upon challenge, a correlation was observed between the in vitro neutralizing capacity and the reduction in severe ECF for individual animals. A protection level upto 85% was obtained. This level of protection was achieved with only two inoculations of 100mug per dose, which is a major improvement over previous recombinant p67 products.

Citation

Vaccine;23(21): 2791-2800

Authors

  • Kaba, S.A.
  • Musoke, A.J.
  • Schaap, D.
  • Schetters, T.
  • Rowlands, G.J.
  • Vermeulen, A.N.
  • Nene, Vishvanath M.
  • Vlak, J.M.
  • Oers, M.M. van

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